INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of palmitoylation during Giardia lamblia encystation
Autor/es:
MERINO C; VRANYCH CV; MIRAS S; TOUZ MC; ROPOLO AS
Reunión:
Congreso; SAIB; 2011
Resumen:
A key step in the life cycle of G. lamblia is the differentiation of the trophozoites into cysts. This process is induced in response to hostspecific factors such as high levels of bile, low levels of cholesterol and a basic pH. One of the early events that occur during encystation is the synthesis of cyst wall proteins (CWP). Post-translational modifications (PTM) such as palmitoylation, are important to regulate molecular mechanisms involved in cell homeostasis and intracellular signaling. It has been shown that Variant-specific surface proteins of G. lamblia are palmitoylated, and the enzyme responsible for this activity has been characterized. However, the role of palmitoylation in encystation has not been reported yet. So, the aim of this work is to elucidate the role of this PTM in encystation in G. lamblia. We observed that when palmitoylation is inhibited by adding 2-Fluoro palmitic acid to the encystation media the percentage of cells expressing CWP1+ decreased. Moreover, we observed an increase of CWP1+ parasites in encysting G. lambliaG. lamblia is the differentiation of the trophozoites into cysts. This process is induced in response to hostspecific factors such as high levels of bile, low levels of cholesterol and a basic pH. One of the early events that occur during encystation is the synthesis of cyst wall proteins (CWP). Post-translational modifications (PTM) such as palmitoylation, are important to regulate molecular mechanisms involved in cell homeostasis and intracellular signaling. It has been shown that Variant-specific surface proteins of G. lamblia are palmitoylated, and the enzyme responsible for this activity has been characterized. However, the role of palmitoylation in encystation has not been reported yet. So, the aim of this work is to elucidate the role of this PTM in encystation in G. lamblia. We observed that when palmitoylation is inhibited by adding 2-Fluoro palmitic acid to the encystation media the percentage of cells expressing CWP1+ decreased. Moreover, we observed an increase of CWP1+ parasites in encysting G. lambliaG. lamblia are palmitoylated, and the enzyme responsible for this activity has been characterized. However, the role of palmitoylation in encystation has not been reported yet. So, the aim of this work is to elucidate the role of this PTM in encystation in G. lamblia. We observed that when palmitoylation is inhibited by adding 2-Fluoro palmitic acid to the encystation media the percentage of cells expressing CWP1+ decreased. Moreover, we observed an increase of CWP1+ parasites in encysting G. lambliaG. lamblia. We observed that when palmitoylation is inhibited by adding 2-Fluoro palmitic acid to the encystation media the percentage of cells expressing CWP1+ decreased. Moreover, we observed an increase of CWP1+ parasites in encysting G. lambliaG. lamblia trophozoites that overexpressed a palmitoyl transferase, compared to wild type G. lamblia. Due to the many intracellular signals involved in the encystation process is possible that PTM such as palmitoylation have an important role. Further studies are needed to find out the molecular mechanisms in which palmitoylation may be involved.G. lamblia. Due to the many intracellular signals involved in the encystation process is possible that PTM such as palmitoylation have an important role. Further studies are needed to find out the molecular mechanisms in which palmitoylation may be involved.