INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Protein Kinase D1 (PKD1)-dependent neurotrophin receptor TrkA trafficking and sorting.
Autor/es:
CORIA, ANDREA SOLEDAD; MASSERONI, MARÍA LUJÁN; DÍAZ AÑEL, ALBERTO MARCELO
Lugar:
Potrero de Los Funes
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2011
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)
Resumen:
After determining the role of several proteins, such as PKD1, in the regulation of intracellular trafficking, we have decided to study the role of these regulatory proteins in the establishment of neuronal polarity, specifically through the analysis of TrkA Neurotrophin Receptor transport and sorting. This receptor is actively localized in the axonal terminal, reaching that specific neuronal region by ligand (NGF)-dependent transcytosis at the dendrites. Without NGF, EGFP-TrkA normal distribution is mostly somatodendritic. In the absence of an active PKD1, using shPKD1 or PKD-KD, we have observed a remarkable decrease in the TrkA distribution at the neuronal processes, together with its accumulation in large vesicles at the neuronal soma. In addition, there was a significant reduction in the length and number of dendrite branches. On the other side, we have analyzed the effect of the lack of PKD1 activity when NGF was present. Even though the length of dendrites was reduced as it was observed without NGF, there was a change in the arborization pattern and in the morphometric variables of dendritic branches. These results would confirm that PKD1 play a key role in the fission regulation of vesicles carrying TrkA, and that the presence of this receptor at the neuronal surface would be essential for dendrite lenght development but not for branching of these neuronal processes.