Protein Kinase D1 (PKD1)-dependent Neurotrophin Receptor TrkA trafficking and sorting.

CORIA, ANDREA SOLEDAD; MASSERONI, MARÍA LUJÁN; DÍAZ AÑEL, ALBERTO MARCELO

Huerta Grande

Congreso; XXVI Congreso Anual Sociedad Argentina Investigación en Neurociencias (SAN); 2011

Sociedad Argentina Investigación en Neurociencias (SAN)

After determining the role of several proteins, such as PKD1, in the regulation of intracellular trafficking, we have decided to study the role of these regulatory proteins in the establishment of neuronal polarity, specifically through the analysis of TrkA Receptor transport and sorting. This receptor is actively localized in the axonal terminal, reaching that specific neuronal region by ligand (NGF)-dependent transcytosis at the dendrites. Without NGF, EGFP-TrkA normal distribution is mostly somatodendritic. In the absence of an active PKD1, we have observed a remarkable decrease in the TrkA distribution at the neuronal processes, together with its accumulation in large vesicles that colocalize with Golgi at the neuronal soma. In addition, there was a significant reduction in the length of dendrite branches. We have also analyzed the effect of inactive PKD1 when NGF was present. Even though the length of dendrites was reduced as it was observed without NGF, there was a change in the arborization pattern and in the morphometric variables of dendritic branches. These results would confirm that PKD1 play a key role in the fission regulation of vesicles carrying TrkA, and that the presence of this receptor at the neuronal surface would be essential for dendrite length development.