INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Participation of sex chromosome complement in the dimorphic expression of brain aromatase
Autor/es:
CISTERNAS CD; CAEIRO X; MIR F; SCERBO MJ; CAMBIASSO MJ
Lugar:
Huerta Grande - Córdoba
Reunión:
Congreso; II Reunión Conjunta de Neurociencias; 2010
Institución organizadora:
Sociedad Argentina de Investigaciones en Neurociencias (SAN) y Taller Argentino en Neurociencia (TAN)
Resumen:
The sexual differentiation of mammalian central nervous system is the result of gonadal steroid action during the critical period (E18-P10) of brain development. Conversion of androgens to estrogens by neural aromatase (ARO) is a prerequisite for brain sexual differentiation. Recent evidences have shown effects of sex chromosome complement (SCC) on sexually dimorphic neural phenotypes. The aim of this study is to evaluate the effect of sex chromosomes on brain ARO expression at E16. To this end, we used the four core genotype mouse model with a deletion of the Sry gene on the Y chromosome (Y−) and a Sry transgene inserted on an autosome. The resulting genotypes are XX and XY− females (ovary-bearing) along with XXSry and XY−Sry males (testes-bearing). Using specific anti-ARO antiserum, the regional distribution was immunohistochemically evaluated in brain sections of E16 mice. ARO-immunorectivity (AROir) were found in the stria terminalis, anterior amygdaloid area, medial and central nuclei of the amygdala. Levels of AROir in the stria terminalis were higher in mice that possessed the SCC XY, regardeless of their gonadal sex. These results suggest that the dimorphic AROir expression in this region is likely controlled mainly by SCC. CONICET y ANPCyT