INIMEC - CONICET 05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
artículos
Título:
Imaging golgi outposts in fixed and living neurons
Autor/es:
CACERES, ALFREDO; CACERES, ALFREDO; BISBAL, MARIANO; BISBAL, MARIANO; QUASSOLLO, GONZALO; QUASSOLLO, GONZALO
Revista:
METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)
Editorial:
Humana Press Inc.
Referencias:
Año: 2016 vol. 1496 p. 31 - 39
ISSN:
1064-3745
Resumen:
Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize GOPs in living neurons.
