Ethanol Induced Neurodegeneration in Bed Nucleus of Stria Terminalis in early Postnatal Periods

BALASZCZUK, V; BENDER, CL; PERENO, G; BELTRAMINO, C

BIOCELL

Biocell

Lugar: Mendoza Argentina; Año: 2007 vol. 31 p. 57 - 57

0327-9545

Studies on the effects of  ethanol  in embrionic and early postnatal periods, described disfunctions in brain development as in proliferation, migration, differentiation, synaptogenesis, and alteration in the developmental process of apoptosis.  Our interest si to study the ontogeny of  the neurons of the Extended Amygdala of the Temporal Lobe, the nerve Growth Factor  and proapoptotic and antiapoptotic mediators expressions, in normal rats and in models of neurodegeneration induced by ethanol. The aim is to analize the neurobiological bases of alterations in the SNC induced by the drug. Methods:  Male rats of 7 and 15 postnatal days (PN),  were used. Control Group: injected with saline  0,9%o sc. Experimental Groups:  Ethanol 20% in saline, sc, 2,5 gr/kg at 0 hour, and 2,5 gr/kg  two hours after.  The Amino-Cupro-Argentic technique (De Olmos et al , 1994) was used to reveal neurodegeneration.  Cells were counted through  a microscope with a Leica DC 200 camera and the KS Lite v2.00  program for statistic analysis. Results: neuronal death was detected as early as 2 hours after ethanol, increasing at 8 and 24 hours in the Lateral Nucleus of the Stria Terminalis of the Central Extended Amygdala, showing its vulnerability to ethanol in this model to study neuroanatomical and neurobehavioral neuropathology in early postnatal periods.