INIMEC - CONICET 05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
artículos
Título:
Receptor-mediated endocytosis and trafficking between endosomal?lysosomal vacuoles in Giardia lamblia
Autor/es:
MARIA R. RIVERO, IGNACIO JAUSORO, MARIANO BISBAL, CONSTANZA FELIZIANI, ADRIANA LANFREDI-RANGEL, MARIA C. TOUZ
Revista:
PARASITOLOGY RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 vol. 112 p. 1813 - 1818
ISSN:
0932-0113
Resumen:
The early branching Giardia lamblia has highly polarized vacuoles, located underneath the plasma membrane, which have at least some of the characteristics of endosomes and of lysosomes. These peripheral vacuoles (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic in trophozoites with a rate similar to the internalization of the low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We speculate on PV functional heterogeneity and maturation in this parasite.
