Presence of neutrophils bearing antigen in lymphoid organs of immune mice.

MALETTO, BELKYS ANGELICA; ROPOLO, ANDREA SILVANA; ALIGNANI DIEGO; LISCOVSKY MIRIAM; RANOCCHIA ROMINA; MORON GABRIEL; PISTORESI MARIA CRISTINA

BLOOD, THE JOURNAL OF THE AMERICAN SOCIETY OF HEMATOLOGY - PRINT

Año: 2006 vol. 108 p. 3094 - 3102

0006-4971

PROOF Abstract 12 During aging, there is an increased rise in susceptibility to infectious diseases. However, it is still unresolved whether standard vaccineDuring aging, there is an increased rise in susceptibility to infectious diseases. However, it is still unresolved whether standard vaccine 13 adjuvants are efficient in the elderly. We report that immunization with OVA plus synthetic oligodeoxinucleotides containing immunostimulatoryadjuvants are efficient in the elderly. We report that immunization with OVA plus synthetic oligodeoxinucleotides containing immunostimulatory 14 CpG motifs (CpG-ODN) stimulates specific Th1 response in aged mice. The immunization with OVA/CpG-ODN induced an increase in CD19+CpG motifs (CpG-ODN) stimulates specific Th1 response in aged mice. The immunization with OVA/CpG-ODN induced an increase in CD19+ 15 cells. These cells from aged mice in vivo captured OVA-FITC as efficiently as those from young mice. Interestingly, naý¨ve aged mice, whichcells. These cells from aged mice in vivo captured OVA-FITC as efficiently as those from young mice. Interestingly, naý¨ve aged mice, which 16 showed Th2 polarization and up-regulation of the expression of GATA-3, with immunization with OVA/CpG-ODN inducing Th1-specificshowed Th2 polarization and up-regulation of the expression of GATA-3, with immunization with OVA/CpG-ODN inducing Th1-specific 17 response but maintaining a Th2 pattern in response to a non-specific stimulator of T cells. Our data suggest that the response elicited by CpG-ODNresponse but maintaining a Th2 pattern in response to a non-specific stimulator of T cells. Our data suggest that the response elicited by CpG-ODN 18 in aged mice have similar properties to the response developed in young mice, emphasizing the importance of the use of this adjuvant duringin aged mice have similar properties to the response developed in young mice, emphasizing the importance of the use of this adjuvant during 19 aging.aging. 20 D 2005 Published by Elsevier Inc.D 2005 Published by Elsevier Inc.