CERZOS   05458
CENTRO DE RECURSOS NATURALES RENOVABLES DE LA ZONA SEMIARIDA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Repetitive sequences survey in Eragrostis curvula cDNA ESTs libraries
Autor/es:
ROMERO JOSE R; JUAN P. SELVA; ECHENIQUE VIVIANA; GARBUS INGRID
Lugar:
San Diego
Reunión:
Congreso; PAG XXII; 2014
Institución organizadora:
PAG XXII
Resumen:
Eragrostis curvula (Schrad) Nees (weeping lovegrass) represents an
important cultivated forage resource in semiarid regions, and the most
useful cultivars are tetraploid and reproduce by pseudogamous
diplosporous apomixis. We previously produced a series of genetically
related E. curvula lines that provide a suitable system for the
identification of gene(s) involved in diplosporous apomixis and ploidy,
including a natural apomictic tetraploid (T), a diploid sexual line (D)
and a tetraploid sexual strain (C). A collection of ESTs was generated
from cDNA libraries obtained from panicles of lines D, T and C, and
leaves of line T. The present study aimed to analyze the repetitive
content of these four cDNA libraries and further identify and
characterize transposable element (TE)-containing ESTs. We found that
the percentage of repetitive content in the leaf cDNA library was almost
double that in inflorescence libraries, with retrotransposons
contributing most in all libraries. The expression of TE-containing ESTs
was compared in cDNA samples extracted from D, T and C leaves or
inflorescences, revealing that six mRNAs containing MuDR-like DNA
transposons, Gyspsy-like and Copia-like retrotransposons were
differentially represented according to tissue, reproductive mode or
ploidy. The present work constitutes the first report on repetitive
sequences of Eragrostis curvula at the transcriptome level