Decay comparison of bacteriophages pp7 and p22 in water matrices, under different environmental conditions: detection by culture-based methods and qPCR

MAIDANA KULESZA, MARIA NOEL; POMA, HUGO RAMIRO; GUTIERREZ CACCIABUE, DOLORES; CORIMAYO, SHEILA; RAJAL, VERÓNICA BEATRIZ

Vienna

Simposio; 20th Symposium on Health-Related Water Microbiology (HRWM); 2019

The International Water Association

Virus behavior in water is usually influenced by different factors. The presence of solid particles is quite significant since they confer protection to them but this will depend on particle type, size and concentration, physicochemical properties of virus, ambient conditions, among others. The aim of this work was to compare the persistence of two phages used as RNA and DNA virus models (pp7 and p22) under different ambient conditions. A group synthetic water matrices were prepared in 500 ml glass beakers using Phosphate Buffer Solution (PBS). A smallest particle size found (