Development of an injectable in situ modified release system for veterinary use: Poloxamer and Polyelectrolytes platforms of release

JOSÉ M. BERMUDEZ; RICARDO GRAU; JUAN CARLO GOTTIFREDI

Rosario

Taller; 1º Taller de Órganos Artificiales, Biomateriales e Ingeniería de Tejidos (BIOOMAT). Sociedad Latinoamericana de Biomateriales, Ingeniería de Tejidos y Órganos Artificiales (SLABO).; 2009

The aim of the present work it is to explore the potential of combining Poloxamer 407 (PO) and polyelectrolytes (PE) as an in situ injectable release system for veterinary use, using Progesterone (Prg) as model drug, and evaluating the release and erosion from the developed formulations. PO, Carragenina (CA), Alginic Acid (AA), Sodium Carboxy methyl cellulose (CM) and Prg were used to prepare the evaluated formulations. To obtain in vitro release profiles; the membraneless model was used at 38 ± 0.05 ºC, with 10 mL of physiological solution as release medium. At predetermined intervals of time, the release medium was completely replaced by new one, which was supported at 38 ± 0.05 ºC. It was determining the weight of the formulations to calculate the proportion of dissolved gel. Prg's release rate is influenced by different ways, according to the PE incorporated in the release platform of PO-Prg. For example, when the gear incorporates CM it increases about a 20%. Whereas the aggregate of AA practically does not influence (3%) and CA's presence decreases the release rate in about 10%. It is noticed that in all the evaluated systems the erosion increases in with the incorporation of the macromolecules in the following order CM ˃AA ˃CA. These results are coincidental with the data of release obtained. PE's incorporation influences significantly in the release and erosion of PO-Prg systems, allowing to select formulations with in vitro optimal properties for the development of an injectable in situ delivery system for veterinary medicine use.