INIQUI   05448
INSTITUTO DE INVESTIGACIONES PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Light sheet fluorescence microscope for fast dynamic imaging
Autor/es:
ROMANO ARMADA, NELI; CALISESI, GIANMARIA; VALENTINI, GIANLUCA; CANDEO, ALESSIA; BASSI, ANDREA
Lugar:
Lewiston
Reunión:
Congreso; Gordon Conference on Lasers in Medicine and Biology; 2018
Resumen:
One of the main challenges in optical imaging is the development of microscopy techniques able to observe fast dynamic processes at high spatial and temporal resolutions, over a large field of view and a long period of time. A microscopy technique that fulfills all these requirements is Light Sheet Fluorescence Microscopy.This poster intends to give a brief presentation of the ideas laying behind volumetric in-vivo optical imaging and how they were implemented in a particular setup.Among the many requirements for this kind of experiment, we highlight the following: 1. High resolution over the entire sample volume;2. High-speed scanning;3. Minimization of optical aberrations;4. Low induced photo damage.As mentioned above, Light Sheet Fluorescence Microscopy grants good optical sectioning thanks to its locally-confined, two-sided illumination.In this poster we illustrate the implementation of a Single Plane Illumination Microscope, including the elements which together grant dynamic imaging: an electrotunable lens, which changes its optical power according to the applied current and a galvanometric mirror, which rotates around an axis at a controllable angular speed.A CMOS sensor was paired with an optical chopper to capture the fluorescence signal excited by light coming from one side or the other of the sample.The use of a programmable board allows us to acquire up to 100 frames per second by achieving a good synchronism between all the aforementioned parts.We display images of genetically marked living samples, acquired with the presented setup.Such frame rates provide for the investigation of in-vivo phenomena occurring over comparable time scales, e.g. calcium dynamics in nerve net recovery or pollutant diffusion in tissues, thus establishing the Single Plane Illumination Microscope as a powerful and adaptive tool.