Poly(3-hydroxybutyrate) membranes for enrofloxacin modified release

DIB ASHUR MARÍA FLORENCIA; ROMERO ANÁLIA I; MERCEDES VILLEGAS; BERMUDEZ JOSÉ M; PARENTIS MÓNICA L

Buenos Aires

Congreso; 6th International Conference on Science and Technology of Composite Materials (COMAT 2016); 2015

Polymeric membranes are being investigated and used in pharmaceutical technology as controlled release systems to modulate drug delivery. Veterinary applications of these systems are mainly focused on meat and milk production, and in prevention and control of pests and diseases in production animals (70%) and pets (30%). Among the materials used to prepare these systems, those that are biodegradable and from natural sources are of growing interest. Polyhydroxyalkanoates, which are a family of biodegradable and biocompatible linear polyesters, have proven to be excellent candidates for medical and pharmaceutical applications. Particular attention has been focused on the use of poly(3-hydroxybutyrate) (PHB). This study evaluated the enrofloxacin release from membranes constituted by PHB. Enrofloxacin is a fluoroquinolone derivative used for treating urinary tract, respiratory and skin infections in animals. Membranes with different concentration of enrofloxacin were synthesized and characterized using Differential Scanning Calorimetry (DSC), Electron Scanning Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). SEM analysis showed the distribution of drug in polymer matrix. DSC and FTIR results indicated physical interaction between drug and polymer. In vitro drug release studies were performed using a pH 5,3 buffer acetate solution. In a test tube, a specific sample of membrane was immersed in this medium and kept stirred at 37C. In different time intervals the medium was removed and immediately replaced with fresh release medium. Enrofloxacin release was evaluated by UV spectroscopy and the profiles were obtained and described using new models. Drug release analysis using our proposed approaches allowed evaluate more accurately the incidence of transport phenomena in drug delivery. Transport and kinetic phenomena involved in drug release processes were both considered in the proposed models.