Preparation and evaluation of enrofloxacin release from poly(3-hydroxybutyrate) membranes

DIB ASHUR FLORENCIA; ROMERO ANALIA; VILLEGAS MERCEDES; BERMUDEZ JOSE; PARENTIS MONICA

BUENOS AIRES

Congreso; 6th International Conference on Science and Technology of Composite Materials; 2015

Asociación Argentina de Ingenieros Químicos

Polymeric membranes are being investigated and used in pharmaceutical technology as controlled release systems to modulate drug release. Veterinary applications of drug delivery systems are mainly focused on meat and milk production, and in prevention and control of pests and diseases in production animals (70%) and pets (30%). One of the main advantages of these systems is the reduction in the frequency of veterinary services and drug administration, increasing the control and prolonging effects, reducing rodeo movements, and reducing stress in animals. This study evaluated the enrofloxacin release from membranes constituted by poly (3-hydroxybutyrate) (PHB). Enrofloxacin is a fluoroquinolone derivative used for treating urinary tract, respiratory and skin infections in animals. PHB is an intracellular polyester synthesized by certain bacteria as a carbon and energy storage compound and shows promising applications in medicine due to its biodegradability and biocompatibility. Membranes with different concentration of enrofloxacin were synthetized and characterized using differential scanning calorimetry (DSC), electron scanning microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). SEM analysis showed the distribution of drug in polymer matrix. DSC and FTIR results indicated physical interaction between drug and polymer. In vitro drug release studies were performed using a pH 5,3 buffer acetate solution. In test tubes, a specific piece of membrane was immersed in this media and kept stirrer at 37ºC. At different time interval the media was remove and instantly replaced by fresh release medium in order to monitoring the progress of the release process. Enrofloxacin release was evaluated with UV spectroscopy and the drug release profile was obtained and described using new models. Drug release analyses using our proposed approaches, allowed evaluate more accurately the incidence of transport phenomena in drug delivery. Transport and kinetic phenomena involved in drug release processes were both considered in the proposed models.