Effects of prunin- and hesperetin glucoside-alkyl (C4-C18) esters interaction with Jurkat cells plasma membrane on membrane physical properties and antioxidant capacity

GUSTAVO CÉLIZ; FEDERICO ALFARO; CECILIA CAPPELLINI; MIRTA DAZ; SANDRA VERSTRAETEN

Buenos Aires

Congreso; VI International Conference on Polyphenols and Health; 2013

Facultad de Farmacia de la UBA

The effects of prunin (P)- and hesperetin glucoside (HG)-alkyl (C4-C18) esters (0.1-100 M) on human leukemia T (Jurkat) cells viability and plasma membrane fluidity were evaluated. After 1 h of exposure, these compounds did not affect cell viability in the range 0.1-10 M. At 100 M, a decrease of cell viability was found that depended on the length of the alkyl chain and that reached a maximum with C6-C12 derivatives. Cell hyperpolarization and shrinkage were also observed at this concentration. The fluidity of cell plasma membrane was not affected, regardless the depths of the membrane level evaluated, although mild changes in plasma membrane hydration were found. The antioxidant capacity of P and HG (0.1-10 M) against 1 mM H2O2 was not affected by esterification. When exposed to 1 mM AAPH, P-alkyl esters retained P antioxidant capacity, but HG-derivatives acted as pro-oxidants. Together, experimental evidences suggest that short term exposures to 0.1-10 M concentrations of P- and HG-alkyl (C4-C18) esters can be considered safe for cultured human cells, and further studies are required to investigate their long term effects, as well their safety for human consumption. Supported by grants of UBA (B086), UNSa and ANPCyT (PI 2009 and PICTO 36683), Argentina.