Biological activity of the lipopeptide-producingBacillus amyloliquefaciens PGPBacCA1 on common beanPhaseolus vulgarisL. pathogens

SABATÉ, DANIELA C.; AUDISIO, M. CARINA; TORRES, M. JULIA; PETROSELLI, GABRIELA; PÉREZ BRANDAN, CAROLINA; ERRA-BALSELLS, ROSA

BIOLOGICAL CONTROL

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2017 vol. 105 p. 93 - 99

1049-9644

Bacillus amyloliquefaciensPGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars ofPhaseolus vulgarisL. were evaluated: cv. Nag (black bean) and cv. Alubia(white bean).Aspergillusspp.,Penicilliumspp. andFusariumspp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibitedCladosporiumspp. and Rhizopusspp., respectively.B. amyloliquefaciensPGPBacCA1 prevented the development of the endophytic fungi of black bean, while onlyCladosporiumspp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition,B. amyloliquefaciensPGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum(76.5%), Rhizoctonia solani(73%), Fusarium solani(56.5%), and Penicilliumspp. (71.5%). The UV-MALDI TOF MS analysis showed thatB. amyloliquefaciensPGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorumandF. solani.These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of FusariumandSclerotinia, respectively. B. amyloliquefaciensPGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent.