INIQUI   05448
INSTITUTO DE INVESTIGACIONES PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Safety assessment of the antimicrobial lipopeptides synthesized by Bacillus subtilis subsp. subtilis CBMDC3f
Autor/es:
BARROSO P.A.; TORRES MARIA JULIA; NOVICOV FANCIOTTI MARIANA; VILLENA JULIO; AUDISIO MARCELA CARINA
Revista:
EC MICROBIOLOGY
Editorial:
ECRONICON
Referencias:
Año: 2017 vol. 8,3 p. 113 - 122
ISSN:
2453-188X
Resumen:
Bacillus subtilis subsp. subtilis CBMDC3f produces lipopeptides with strong antibacterial activities that were previously identified by UV-MALDI-TOF mass spectrometry as different surfactin homologues. In this work, the potential toxic effects of those lipopeptides were explored on BALB/c mice. The following samples of CBMDC3f antimicrobial metabolites were evaluated: cell-free supernatant (CFS) and purified lipopeptides fractions (LF), at different concentrations: 25, 125 and 250 mg/Kg of body weight (LF25, LF125 and LF250 groups, respectively). As a measure of hepatocellular toxicity, the serum enzymes (GOT and GPT transaminases) concentrations and liver and intestine histological analyses were performed. Values of GOT and GPT enzymes did not reveal significant changes as a result of the treatments. The histological analysis showed some signs of liver injury when CFS was administered; however, the liver injury in CFS-treated mice was significantly milder than those observed in the LF250 group. CFS, LF25 and LF125 treatments did not produce histologic changes in the small intestine. These results indicate that surfactins present in the LF of B. subtilis subsp. subtilis CBMDC3f would not produce cytotoxic effect on liver or small intestine of mice when administered at concentrations between 25 and 125 mg/Kg of body weight. Considering that the concentrations of CBMDC3f lipopeptides able to exert the anti-Listeria monocytogenes and anti-Staphylococcus activities are significantly lower than those with toxic effects; the results of this work indicate that CBMDC3f lipopeptides are promising for their application in food protection.