INIQUI   05448
INSTITUTO DE INVESTIGACIONES PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent
Autor/es:
D. ROYON, M. DAZ, G. ELLENRIEDER, S. LOCATELLI
Revista:
BIORESOURCE TECHNOLOGY
Editorial:
Elsevier
Referencias:
Año: 2007 vol. 98 p. 648 - 653
ISSN:
0960-8524
Resumen:
The enzymatic production of biodiesel by methanolysis of cottonseed oil was studied using immobilized Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The eVect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24 h at 50 °C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95% ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The eVect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24 h at 50 °C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95% ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The eVect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24 h at 50 °C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95% ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The eVect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24 h at 50 °C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95% ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.Vect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24 h at 50 °C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95% ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95% ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.Wxed bed continuous reactor with a Xow rate of 9.6ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields. © 2006 Elsevier Ltd. All rights reserved.2006 Elsevier Ltd. All rights reserved.