Evaluation of detachment methods for the enumeration of Bacteroides fragilis in sediments via propidium monoazide-quantitative PCR, in comparison to Enterococcus faecalis and Escherichia coli?

MINJI KIM; DOLORES GUTIÉRREZ CACCIABUE; ALEX SCHRIEWER; VERÓNICA RAJAL; STEFAN WUERTZ

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2014 vol. 117 p. 1513 - 1522

1364-5072

Aims: The aim was to develop an optimized detachment method for separating Bacteroidales from sediments to allow enumeration via PMA-qPCR. The effectiveness of four different detachment treatments in removing Bacteroides fragilis was compared as a function of time as well as in relation to Enterococcus faecalis and Escherichia coli as detected by cultivation and qPCR.  Methods and Results: Cells were inoculated into four sediments from seawater (SW) and freshwater (FW) beaches. Sediment samples were taken on days 1 and 7 and subjected to four different treatments for separation of microorganisms. On day 1, the detachment treatments performed equally well in removing intact B. fragilis cells. In contrast, 7 days later the detachment treatment with Tween 80 and handshaking (TH) resulted in up to 8-fold higher 16S rRNA gene concentrations of intact and total B. fragilis cells compared to other detachment treatments. Total E. faecalis cells based on the 23S rRNA gene were also preferentially recovered by treatment TH. Cultivable E. faecalis or E. coli numbers detached from sediments were similar for all methods in SW and FW sediments. Conclusions: Handshaking and 1% Tween 80/NaOH (pH 7.0) eluant was the most efficient technique to recover intact as well as total B. fragilis cells in sediment samples with different salinities and after prolonged sediment-cell contact time. Significance and Impact of the Study: The optimized detachment method enables the application of PMA-qPCR to sediment samples to detect the presence of Bacteroidales cells and their DNA in future microbial source tracking studies.