INSTITUTO DE INVESTIGACIONES EN ENERGIA NO CONVENCIONAL
Unidad Ejecutora - UE
congresos y reuniones científicas
An automatic wrapped phase gradient-based method for spherical aberration correction in Digital Holographic Microscopy
A. V. BLANC; G. G. ROMERO; A. C. MONALDI; C. M. CABRERA
Conferencia; IX Conference RIAO / OPTILAS 2016; 2016
Most Digital Holographic Microscopy architectures introduce a Microscope Objective in the object arm of the micro-interferometer in order to increase lateral resolution but, as a consequence, a spherical phase aberration arises. The phase distortion must be corrected to achieve reliable phase information linked to the microscopic object under study. In this work, we present a fast and simple numerical method for automatically compensate the phase curvature from the wrapped phase gradient of the reconstructed hologram. The main advantage is that, as non-unwrapping methods are required, it avoids any intrinsic errors of these methods. Furthermore, no additional holograms recording are needed and does not required prior knowledge of the object or the setup. The method is experimentally demonstrated by a phase contrast imaging of a Pediastrum cell.