INENCO   05446
INSTITUTO DE INVESTIGACIONES EN ENERGIA NO CONVENCIONAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Digital Holographic Microscopy for Detection of Trypanosoma Cruzi parasite in Blood
Autor/es:
A. C. MONALDI; G. G. ROMERO; E. E. ALANÍS
Reunión:
Congreso; VII Reunión Iberoamericana de Óptica, X Encuentro Latinoamericano de Óptica, Láseres y Aplicaciones; 2010
Resumen:
The potentialities of the Digital Holographic Microscopy for the detection of livingmicroorganisms in blood samples are researched in this work. A thin layer of fresh blood infectedby the protozoan Trypanosoma cruzi is subjected to analysis in order to detect the presence of theparasites in a microscopic vision field. The proposed technique is based on the measurement ofphase distributions corresponding to temporal differentiation in sequences of digital holograms ofthe same vision field. The presence of parasites is detected from characteristic pattern of the movingparasites observed in the amplitude and phase differences. A comparison of different methods ofnumerical reconstruction, and a discussion about the optimal temporal lag between holograms arepresented. The purpose of the work is to optimize an automated opto-mechanical system aimed atChagas disease diagnostic.
rds']