Probiotic lactobacillus reinforce the intestinal epithelial barrier.

PERDIGÓN, GABRIELA.; BALCELLS, FLORENCIA; CAZORLA, SILVIA INES; MARTINEZ MONTEROS, MARIA JOSÉ; MALDONADO GALDEANO, CAROLINA

Buenos Aires

Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2020

SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)

The epithelial layer not only serves as a passive physical barrier with external environment. Intestinal epithelial cells sense external or endogenous danger signals and mount a robust immune response to maintaining intestinal homeostasis. We previously demonstrated that lactobacillus increase Paneth cells and intestinal antimicrobial activity in order to keep this balance. Our aim was to further analyzed the effect of probiotic lactic bacteria on the intestinal epithelial barrier (IEB). Mice were fed with Lactobacillus casei CRL 431; Lactobacillus paracasei CNCM I- 1518 for 7 and 5 days, respectively, or water. On day 8 mice were killed and samples were removed.Mice fed with the probiotics increased ex vivo intestinal antimicrobial activity against Staphylococcus aureus, Salmonella thyphimurium and a multi-resistant drugs Klebsiella pneumoniae. This correlates with high levels of the defensin-α5 in the intestinal fluids (p