CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The nasal administration of Lactobacillus rhamnosus promotes the recovery of lung B cells affected by malnutrition
Autor/es:
BARBIERI NATALIA; SALVA SUSANA; HERRERA MATÍAS; VILLENA JULIO, ALVAREZ SUSANA
Lugar:
Buenos Aires, Argentina
Reunión:
Congreso; First French - Argentine Immunology Congress. LVIII Reunión Anual de la Sociedad Argentina de Inmunología. XIII Jornada Científica del Grupo Rioplatense de Citometría de Flujo. 3º Jornadas Argentinas de Inmunodeficiencias Primarias (SAP); 2010
Institución organizadora:
Sociedad Argentina de Inmunología. Grupo Rioplatense de Citometría de Flujo
Resumen:
The nasal administration of Lactobacillus rhamnosus CRL1505 (Lr05) is able to improve the innate and specific immune responses against Streptococcus pneumoniae in malnourished mice. In this work, we analyzed the effect of the nasal administration of Lr05 on the recovery of lung B cells during a repletion treatment. Weaned mice were malnourished after consuming a protein-free diet (PFD) for 21d. Malnourished mice were repleted with a balanced diet (BD) for 7d or BD 7d with Lr05 nasal administration (108cells/mouse/d) on d 6 and 7 (BD+Lr05). The malnourished control mice (MC) received PFD while the well-nourished control group (WC) consumed BD. At the end of the treatments, we evaluated: body weight, total number of lung cells and the B cell population of lung by flow cytometry (CD19, B220, HSA, IgM, IgD). The MC mice showed lung cell counts significantly lower than the WC. Both repletion treatments increased lung cells but only the BD+Lr05 mice reached higher values than the WC group (p<0.05). Malnutrition decreased lung lymphocytes (FSC vs SSC) and BD+Lr05 tretament was able to normalize this parameter (WC=9.71±1.8; MC=3.81±0.39; BD=6.78±1.6; BD+Lr05=10.9±1.17 105 cells/mouse). The MC group showed a significant decrease of B cells in lung compared to WC mice (p<0.05); due to the decrease in the number of mature (B220highHSAlowIgM+IgD+) and immature (B220low HSAhighIgM+IgD-) B cells. The treatment with Lr05 induced an improvement in the number of lung mature B cells (WNC=1.38±0.2; MNC=0.38±0.02; BD=0.89±0.2; BD+Lr05=1.99±0.21 105cells/mouse). On the contrary, BD treatment failed to normalize this parameter. In addition, both repletion treatments induced the normalization of immature B cells. This work demonstrates that the nasal administration of Lr05 during the repletion diet promotes the recovery of lung B cells. This effect on B cell would explain the ability of immunobiotic Lr05 to improve the specific immune response against S. pneumoniae in malnourished mice.