Lactobacillus salivarius TUCO-L2 ISOLATED FROM Lama glama MILK DIFFERENTIALLY MODULATE TOLL-LIKE RECEPTOR 4-MEDIATED INNATE IMMUNITY IN PORCINE INTESTINAL EPITHELIAL CELLS

MANSILLA, F.; ALBARRACÍN, L.; KITAZAWA, H.; TAKAGI, M.; TAKAHASHI, H.; QUILODRÁN-VEGA, S.; ASO, H.; VILLENA, J.

SAN MIGUEL DE TUCUMAN

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Lactobacillus salivarius TUCO-L2, previously isolated from Lama glama milk, was selected according to its ability to inhibit Gram negative gastrointestinal pathogen´s growth in mice models. In this work, the ability of TUCO-L2 to differentially modulate the innate immune response triggered by TLR4 activation in porcine intestinal epithelial (PIE) cells was evaluated. PIE cells were stimulated with the TUCO-L2 strain (5×108 cells/ml) for 48 h and then, challenged with heat-stable pathogen molecular associated patterns (PAMPs) from enterotoxigenic Escherichia coli (ETEC) to induce the activation of TLR4. Twelve hours after the challenge, the expression of cytokines (IL-, IL-6), and chemokines (IL-8, CXCL5, CXCL9, CXCL10, CXCL11, CCL8) were evaluated by qPCR. In addition, the expressions of the following negative regulators of TLR4 signaling were studied: A20, Bcl-3, IRAK-M, MKP-1, SIGIRR, and Tollip. All the pro-inflammatory factors evaluated were increased in PIE cells after TLR4 activation. However, L. salivarius TUCO-L2-treated PIE cells showed significantly reduced expression of IL-8, CXCL5, CXCL9, CXCL10 (p