CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Potential effect of Lactobacillus casei on platelets and endothelium activation in an infection model
Autor/es:
HARO C; ZELAYA H; LAIÑO J; ALVAREZ S; AGÜERO G
Lugar:
San Miguel de Tucumán. Tucumán. Argentina
Reunión:
Simposio; III Simposio Internacional de II Bacterias Lácticas y Segundo Encuentro de la Red Argentina de Bacterias Lácticas (Red-BAL).; 2009
Institución organizadora:
Centro de Referencia para Lactobacilos - CERELA- CONICET
Resumen:
Local and systemic inflammation can produce a prothrombotic environment driven by tissue factor, adhesion molecules, pro-inflammatory cytokines and prothrombotic microparticles. We previously demonstrated that Lactobacillus casei supplementation reduced the coagulation abnormalities caused during a respiratory infection model in mouse. We evaluated the effect of the oral administration of Lactobacillus casei CRL 431 (Lc) on the platelets and endothelial activation during a Streptococcus pneumoniae (Sp) infection in mice. Swiss Albino mice received during 2 consecutive days (d) Lc (Lc group) in the drink water (109cells/day/mouse). At the end, mice with and without treatment (C group) were challenged intranasally with Sp. At different days post-infection (dpi) we determined: I-a) von Willebrand factor (vWF), indicator of endothelial activation, in plasma and bronchoalveolar lavage (BAL), b) expression of vascular cell adhesion molecule 1 (VCAM-1) in lung histological slice, c) expression of chemokine receptor CXCR4 in leukocytes. II- platelets were identificated by CD41 expression and the degree of platelets activation by P-selectin (CD 62-P) expression on whole blood by flow cytometry. The infection induced increase of vWF in BAL, VCAM-1, CXCR4 and CD 62-P expression in both groups. The mice treatment with Lc showed lower vWF levels in BAL (vWF BAL5dpi: C=39,0±3,6 Lc=11,5±3,5 %) and VCAM-1 expression than C group, and increase CD 62-P expression in platelets (CD 62-P1dpi: C=1,0±0,3 Lc=2,8±0,2 %) until the end of the experiment. Mice that received the lactic bacterium evidenced lower CXCR4 expression on neutrophils and monocytes than C group before infection, after that the behavior was similar in both. On the basis of the obtained results it is possible concluded that Lc supplementation was effective to regulate the endothelial and platelets. Also the higher P-selectin expression would be beneficial for the host because activated platelets release growth factors that favor the vascular repair.