Lactobacillus reuteri Reduces Apoptosis and ERK 1/2 Activity in PBMC with Disrupted Rafts

MECHOUD, M.A.; FONT DE VALDEZ, G.; RODRIGUEZ, A.V.

San Miguel de Tucumán, Tucumán, Argentina

Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2009

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} a:link, span.MsoHyperlink {color:blue; text-decoration:underline; text-underline:single;} a:visited, span.MsoHyperlinkFollowed {color:purple; text-decoration:underline; text-underline:single;} @page Section1 {size:595.3pt 841.9pt; margin:70.9pt 3.0cm 70.9pt 3.0cm; mso-header-margin:35.45pt; mso-footer-margin:35.45pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Lactobacillus reuteri REDUCES APOPTOSIS AND ERK 1/2 ACTIVITY IN PBMC WITH DISRUPTED RAFTS Mechoud MA, Font de Valdez G, Rodriguez AV. CERELA-CONICET. Chacabuco 145. CPA (T4000ILC). Tucumán, Argentina. E-mail: mmechoud@cerela.org.ar   The immunomodulatory capacity of lactobacilli were previously studied but the molecular mechanisms involved in this effect remain unclear. Our previous studies showed that L. reuteri CRL 1098 reduced TNF-α production by human peripheral blood mononuclear cells (PBMC), and this effect depended on lipid rafts integrity. The aim of this work was to investigate the apoptotic effect of L. reuteri on control and disrupted-rafts PBMC (dr-PBMC) and the modulation of ERK1/2 and p38 activities. Also, IL-10 regulation by L. reuteri was investigated in order to determine the anti-inflammatory capacity of this strain. Kinases activities and Il-10 were detected by ELISA and apoptosis by flow cytometry. When PBMC were co-incubated with L. reuteri, no modification on IL-10 production was observed. These results, with the previous one of diminished TNF-α level, indicate that L. reuteri exerts an anti-inflammatory effect on PBMC. Apoptosis was reduced by 5 and 3% in control and dr-PBMC respectively, when cells were co-incubated with L. reuteri. No modifications on p38 activity neither in control or dr-PBMC were induced by L. reuteri. In contrast, ERK1/2 activity was decreased, indicating that this signaling pathway may be involved in the cellular response to L. reuteri. These results provide clues to understand the mechanisms by which L. reuteri produces an anti-inflammatory effect on PBMC.