CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ADVANCES IN THE DESIGN OF ONE UROGENITAL PROBIOTIC FORMULA: COMPATIBILITY, ANTIMICROBIAL SUSCEPTIBILITY AND RESISTANCE TO LYOPHILIZATION OF LACTOBACILLI
Autor/es:
VERA PINGITORE E.; JUÁREZ TOMÁS M. S.; NADER-MACIAS M.E.
Lugar:
Tucumán - Argentina
Reunión:
Simposio; III Simposio Internacional de II Bacterias Lácticas - Segundo Encuentro de la Red Argentina de Bacterias Lácticas (Red- BAL); 2009
Institución organizadora:
CERELA - CONICET (Centro de Referencia para Lactobacilos - Consejo Nacional de Investigaciones Científicas y Técnicas )
Resumen:
Urogenital infections in women are associated with high morbidity and mortality rates as well as with high health care costs. A probiotic formula to control these situations can include one microorganism or a mixture of strains, with particular properties, and other non-probiotic active ingredients (vitamins, prebiotic substances, hormones, etc.). The objectives of the present study was to determine in vaginal lactobacilli (VL): a) their interaction degree (compatibility), b) their antimicrobial susceptibility in order to differentiate compatible VL in the mixtures, and c) their behavior during freeze-drying process and storage in presence of A vitamin.The compatibility of 13 vaginal lactobacilli, previously selected for their potentially probiotic properties (inhibition of growth of uropatogens by antimicrobial substances and/or remarkable auto-aggregating pattern) was studied according to the plate diffusion method in LAPTg agar, by employing each microorganism both as indicator and producer of inhibitory substances. Susceptibility to 13 antimicrobial agents was studied by employing the disc diffusion method. Minimal Inhibitory Concentration (MIC) with seven different antibiotics was determined by the plate dilution assay. Freeze-dried Lactobacillus spp. CRL (from the CERELA Culture Collection) 1263 and Lactobacillus spp. CRL 1252, both as single and mixed cultures, were combined with A vitamin, placed into gelatin capsules and stored at different temperatures (7°C and 25°C).Most VL strains (69%) inhibited the growth of microorganisms of the same genus and ecological origin. According to compatibility results, five VL were selected to determine their antimicrobial susceptibility. VL did not show significant differences in the antimicrobial resistance profiles determined through the disc diffusion method. However, the MICs have shown that a growth medium containing 0.05 µg/ml de erythromycin allows to perform a differential viable cell count of Lactobacillus spp. CRL 1252 and Lactobacillus spp. CRL 1263. Survival of VL after lyophilization and storage for 5 months was affected at different extents depending on strains, temperature storage and A vitamin added to the freeze-dried powders. Lactobacillus spp. CRL 1263 was the most resistant strain to the freeze-drying and storage at 7°C. For both freeze-dried VL, either as single or mixed cultures, no viable cells were detected after 5 months of storage at 25°C.Several potentially probiotic VL are compatible between them, and the resistance to antimicrobial agents and to lyophilization are strain-dependent characteristics. This study represents an advance in the design of a pharmaceutical formula containing compatible microorganisms to prevent or treat urogenital infections in women.