Effect of yoghurt administration on recurrent intestinal inflammation model

CHAVES, S; PERDIGÓN G; DE MORENO DE LEBLANC, A

San Miguel de Tucumán, Argentina

Simposio; III Simposio Internacional de Bacterias Lácticas. II Encuentro red BAL Argentina; 2009

CERELA

Inflammatory bowel disease may result from exaggerated stimulation of the mucosal immune system by luminal bacterial biota. It was reported the efficacy of the yogurt (Y) against IBD but their mechanisms of action are unknown. In previous studies we demonstrated that continuous administration of Y was able to regulate the inflammatory response in a acute intestinal inflammation model induced by 2,4,6 trinitrobenzene sulfonic acid (TNBS). In the present study, we investigated the effect of the Y administration to mice with TNBS-induced recurrent colitis. After 7–9 days (d), mice inoculated with TNBS that recovered their initial weight were divided into two groups. One group received Y ad libitum during 21 d, and the other group received water and conventional food ad libitum, at the end of feeding period the samples were taken. The remaining mice of each group received a 2nd intrarectal administration of TNBS. The large intestine (LI) and liver were removed 3d after the 2nd TNBS administration to evaluate liver translocation (LT), colon histology and intestinal microbiota. Weight changes and mortality were also evaluated. The recurrent control group showed the highest weight loss 2 and 3 days after the 2°TNBS administration compared with the mice that received Y administration in the same periods of time. The mortality rate shown significantly differences compared the recurrent control group with the mice given Y (without mortality). The control recurrent group showed the most important changes in the structure of large intestine and the highest score of colonic damage. Mice fed 21 d with Y showed a significant increase in the bifidobacteria counts 3 d after of 2° TNBS administration, compared to the control recurrent group without Y. Enterobacteria population diminished in mice that received Y compared with control recurrent group. For other bacterial population, differences were not observed. LT did not show differences between control and Y feeding group, 21d after 1° TNBS administration. TNBS-TNBS group increased the percentage of mice with LT evaluated in the three different media (MacConkey, LAPTg and MRS). Mice from TNBS-Y-TNBS group showed lower percentage of translocation than the TNBS-TNBS group. We demonstrated that yoghurt administration was able to attenuate the symptoms of recurrent intestinal inflammation, and reduced the LT, probably by desirable changes observed in the intestinal microbiota.