Food prospects of selenium enriched-Lactobacillus acidophilus CRL 636 and Lactobacillus reuteri CRL 1101

TERESA, PEREZ-CORONA; MICAELA PESCUMA; FERNANDA MOZZI; GRACIELA FONT DE VALDEZ; BEATRIZ GOMEZ GOMEZ; YOLANDA, MADRID

Egmond aan Zee

Simposio; 12th International Symposium of Lactic Acid Bacteria; 2017

Food prospects of selenium enriched-Lactobacillusacidophilus CRL 636 and Lactobacillusreuteri CRL 1101MicaelaPescuma1,2, Beatríz Gomez-Gomez2, Teresa Perez-Corona2,Graciela Font1, Yolanda Madrid2*, Fernanda Mozzi1*1Centro de Referencia paraLactobacilos (CERELA)-CONICET. Chacabuco 145. 4000 Tucumán, Argentina. 2Departamentode Química Analítica. Facultad de Ciencias Químicas, Universidad Complutense deMadrid. Ciudad Universitaria. 28040. Madrid. Spain. Selenium (Se) is an essential trace element present as SeCys in proteins (selenoproteins) involvedin antioxidant functions, thyroid hormone production, viral expression genes,etc. Normally, animals incorporate Se viaplant ingestion. Due to low vegetable consumption Se deficiencies in humans havebecome a health problem. Additionally, Se has to be consumed as elemental Se(Se0) or as organic forms to be non-toxic. Some lactic acid bacteriacan produce Se0 nanoparticles and selenoproteins. In this work, weaimed to study the cell behavior and the ability to accumulate andbiotransform Se by Lactobacillusacidophilus CRL 636 and L. reuteriCRL 1101 in the presence of selenite. Moreover, the capability of the strainsto survive in the presence of bile salts and at different pH values (4.0 and8.0) after Se enrichment was evaluated. Both strains were able to grow, producenanoparticles and accumulate Se, being L.reuteri CRL 1101 the strain that accumulated the highest amount (78 % at 24h) of Se. Interestingly, SeCys was found in cell pellets of both strains. TheSe-enriched strains showed lower growth rate when transferring them in controlmedium; this effect was greater when subjecting them to the presence of Seagain, indicating that the strains could not adapt to the presence of Se. Incontrast, the resistance of Se-enriched L.reuteri CRL 1101 to bile salts was improved. On the whole, results indicatethat these strains could be administrated directly as probiotics or used themfor formulating fermented products containing Se enriched bacteria andnanoparticles.