Pasta elaborated with quinoa sourdough fermented with selected strains of lactic acid bacteria is able to prevent mineral, folate and riboflavin deficiencies in vivo

L. CARRIZO; G. ROLLAN; J.G. LEBLANC; A. DE MORENO DE LEBLANC

Egmond aan Zee

Congreso; 12th International Symposium on Lactic Acid Bacteria; 2017

Royal Netherlands Society for Microbiology (KNVM) and the Federation of European Microbiological Societies(FEMS)

Quinoa (Chenopodium quinoa Willd) is an Andean ancestral crop currently revalued for iis high nutritional value, contains vitamins and minerals and all essential amino acids. The bioavailability of these compounds is limited by antinutritional factors such as phytic acid (FA) which can be hydrolyzed by the phytase enzyme. Folate (B9) and riboflavin (B2) are important cofactors of metabolic enzymes in the cell. Due to the deficiency of these vitamins in the world, food fortification programs have been developed. An attractive alternative is the use of lactic acid bacteria (LAB) because of their adaptability to fermentation processes, their biosynthetic capacity and their metabolic versatility. LAB are ideal candidates for the in situ production of B9, B2 and phytases in food. The objective of this study was to evaluate the effectiveness of pasta made with quinoa fermented by L. plantarum strains that produce B9, B2 and phytase to prevent vitamin and mineral deficiency in a mouse model. The results showed that bio-enriched pasta in vitamins and minerals increased levels of B9 and B2 in blood. Likewise, higher concentrations of P, Ca, Fe2 , Mg2 * (11 .85, 9.90, 0.26, 3.34 mg/dl, respectively) were determined with respect to the deficient group (18.75-10.70-0, 36-4.65.mg/dl). Hematological studies showed an increase: 1 .5; 9.0 and 1 3.0% in red blood cells, hemoglobin, and hematocrit, respectively in relation to the deficient group in which hypochromia and multi-segmented nuclei were observed in neutrophils. Histological sections of the intestine showed an increase (approximately 50%) in the length of intestinal villi. The results allow us to conclude that bio-enriched quinoa paste could be considered as an effective and viable strategy in the prevention of deficiency of viiamins and minerals.