CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Sesquiterpene lactones from Mikania species display in vitro activity against trypomastigotes and amastigotes of Trypanosoma cruzi
Autor/es:
SÁNCHEZ ALBERTI A; GROSSO C; ALONSO MR; LAURELLA L; BIVONA A; CATALAN C; SULSEN V; CERNY N; MALCHIODI EL; CAZORLA SI
Lugar:
CABA
Reunión:
Simposio; XVIII Simposio Internacional sobre Enfermedades Desatendidas; 2017
Institución organizadora:
Mundo Sano
Resumen:
Introduction: Chagas´ disease is a parasitic disease caused by the protozoan Trypanosoma cruzi. According to the World Health Organization (WHO) it is considered, among others, a Neglected Tropical Disease (NTD) affecting mainly poor people. Actually, available drugs to treat Chagas disease are not sufficiently effective and have severe side effects. Therefore, and because of the growing global health problem generated by this disease there is an urgent need to find new drugs for its treatment. In this sense, natural products due to their wide chemical diversity constitute an important source of bioactive compounds. The genus Mikania belongs to the Asteraceae family. In the last decades, the family Asteraceae has been studied due to the quantity and variety of active compounds produced by its members. Some of the most representative compounds with antiparasitic activity are sesquiterpene lactones such as artemisinin, parthenolide, psilostachyins and cynaropicrin, among others.We have previously reported the antiprotozoal activity of two sesquiterpene lactones, mikanolide and dihydromikanolide, isolated from Mikania micrantha and Mikania variifolia. In this sense, the aim of the present work was to evaluate these compounds against trypomastigotes and amastigotes of T. cruzi. Material and methods: The trypanocidal effect of the compounds was tested on bloodstream trypomastigotes and the effect of each compound on intracellular forms of T. cruzi was assayed using β- galactosidase transfected parasites. Results: Mikanolide and dihydromikanolide were active against Trypanosoma cruzi bloodstream trypomastigotes with 50% inhibitory concentrations (IC50) of 2.1 and 0.3 µg/ml and against amastigotes with IC50 values of 4.5 and 8.5 µg/ml, respectively.Discussion: Based on the results obtained we can conclude that mikanolide and dihydromikanolide are promising compounds and future biochemical assays will be conducted in order to determine their mechanism of action and their in vivo activity.