CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
RELEASE OF COBALAMIN BY Lactobacillus coryniformis CRL 1001 DURING THE TRANSIT THROUGH THE GASTROINTESTINAL TRACT
Autor/es:
CAROLINA TORRES; FONT DE VALDEZ G; MARÍA INÉS TORINO; MARÍA PÍA TARANTO; MAGDALENA AVILA; L. SAAVEDRA
Lugar:
San Miguel de Tucumán
Reunión:
Simposio; International Symposium on Lactic Acid Bacteria; 2016
Institución organizadora:
CERELA-CONICET
Resumen:
Cobalamin is synthesized only by some bacteria and archaea and is essential for humans whoabsorbed this vitamin by an intestinal mechanism using the intrinsic factor, a protein producedby the stomach cells. Previously, we demonstrated that Lactobacillus (L.) coryniformisCRL 1001 genome has at least 30 genes involved in the cobalamin biosynthesis (cbi-cob-hemgenes) and the cell extract of this strain is able to correct the coenzyme B12 requirements ofSalmonella enteric serovar Typhimurium AR 2680 in a minimal medium. These findings werethe first evidence for cobalamin biosynthesis de novo in this species of Lactobacillus. The aimof this study was to evaluate the survival and cobalamin released by lysed cells of CRL 1001strain during the exposition to gastrointestinal artificial conditions. A decrease in the cellularviability was observed in the presence of artificial solutions similar to saliva, gastric juice andpancreatic juice. In the presence of supernatant of gastric and pancreatic juice inoculatedwith the L. coryniformis CRL 1001, the AR 2680 strain showed growth halos. These resultswould bring out that the CRL 1001 strain would be partially lysed during the gastrointestinalpassage releasing cobalamin. These results support the idea of using this strain for the developmentof functional foods for people with vitamin B12 deficiency.