CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biocontrol of Listeria monocytogenes FBUNT biofilm developed on industrial surfaces.
Autor/es:
MELIAN CONSTANZA; SEGLI FRANCO; CASTELLANO PATRICIA; VIGNOLO GRACIELA
Lugar:
san miguel de tucuman
Reunión:
Simposio; V Simposio Internacional de Bacterias Láctica.; 2016
Resumen:
BIOCONTROL OF LISTERIA MONOCYTOGENES FBUNT BIOFILM DEVELOPED ON INDUSTRIAL SURFACESF. Segli1, C.V. Melián1, G. Vignolo1, P. Castellano1 1Centro de Referencia para Lactobacilos, CERELA-CONICET, Chacabuco 145. 4000 Tucumán, Argentina. E_mail: cmelian@cerela.org.arAdhesion of pathogenic and/or spoilage microorganisms and biofilm development on equipment materials constitute a potential chronic source of microbial contamination threatening the safety and quality of food products which result in food-borne disease and economic losses. L. monocytogenes demonstrated a remarkable ability to colonize and persist on food-processing environments including refrigerated premises. Recently, major advances have been produced in the prevention and control of pathogens biofilm by lactic acid bacteria (LAB). Therefore, the objective of this study was to investigate the ability of Lactobacillus curvatus CRL705, L. curvatus CRL1532 and/or their bacteriocins to displace L. monocytogenes FBUNT during biofilm formation on stainless steel (SS) and polytetrafluoretylene (PTFE) surfaces at 10 °C. Inhibitory strategies involved (i) L. curvatus CRL705 or CRL1532 planktonic cells and (ii) their partially purified bacteriocins on pathogen biofilm grown in Luria-Bertani medium during 6 days at 10 °C. As a result planktonic cells of both LAB as well as bacteriocins treatment were able to displace the pre-established pathogen biofilm after 30 min of contact Moreover, a greater inhibition of L. monocytogenes biofilm on SS than on PTFE was found. Reductions of pre-existing biofilms of L. monocytogenes between 2.0 ± 0.13 and 2.5 ± 0.15 log cycles were observed after both treatments (planktonic cells or bacteriocins of L. curvatus CRL705 and CRL1532) on SS surfaces. By Scanning electron microscopy (SEM), a similar pattern of inhibition was observed for both antimicrobial treatments tested. These results show the effectiveness of bacteriocinogenic strains, L. curvatus CRL705 and L. curvatus CRL1532, and their bacteriocins for the prevention and control of L. monocytogenes on SS as abiotic surfaces.