Bacteriocins of meat isolated lactobacilli strains impair the adhesion and invasion of Listeria monocytogenes FBUNT in Caco-2 cells

CASTELLANO, PATRICIA; NIÑO ARIAS, FABIÁN CAMILO; DE MARTINIS, ELAINE CRISTINA; PÉREZ IBARRECHE, MARIANA; SOUZA, VANESSA MACIEL; LONGO BORGES, LILIANA; DE SOUSA, LUCAS

Tucumán

Simposio; V Simposio Internacional de Bacterias Láctica.; 2016

CERELA

Listeria monocytogenes is an ubiquitous bacterium that causes listeriosis, a rare foodborne disease that affects severely immunocompromised patients, pregnant women, children and the elderly. Its control is a challenge for the food industry due to its persistence even in hostile environments. Lactic acid bacteria (LAB) may produce antimicrobial peptides (bacteriocins) active against L. monocytogenes, with some proposed food applications. However, the role of bacteriocins to prevent adhesion and invasion of intestinal cells by L. monocytogenes is not well understood. In this study, antilisterial activities of bacteriocin-containing cell-free culture supernatants (CFSs) from Lactobacillus curvatus CRL705, CRL1532, CRL1533 and Lactobacillus sakei CRL1613 were evaluated in eukaryotic cell model of adhesion and invasion (Caco-2). All CFSs were active against L. monocytogenes FBUNT, in all concentrations tested (two per strain): 2880 and 1600AU/ml for L. curvatus CRL705; 1440 and 800AU/ml for L. curvatus CRL1532; 720 and 400AU/ml for L. curvatus CRL1533; 720 and 400AU/ml for L. sakei CRL1613. On average, inhibition of L. monocytogenes adhesion to Caco-2 cells was 3.56±0.13 log CFU/ml for all CFSs of lactobacilli tested. With regard to the invasion ability of L. monocytogenes, the highest concentrations of bacteriocins tested were the most effective. Highest concentrations of CFSs of L. curvatus CRL705 and L. curvatus CRL1532 inhibited completely the invasion of Caco-2 cells by L. monocytogenes. These results encourage further studies on the potential health benefits of these lactobacilli strains.