Antagonistic effect against Salmonella of poultry origin strains evaluated in a natural medium of cecal water

FERNÁNDEZ MARÍA MAGDALENA; QUIROGA M.; APELLA M. C.; ARGAÑARAZ-MARTÍNEZ ELOY

San Miguel de Tucumán

Simposio; V International Symposium on Lactic Acid Bacteria SIBAL2016; 2016

CERELA-CONICET

The salmonellosis, caused by different serotypes of Salmonella, is an important concern to the avian industry because of the production loss, increased mortality, and the associated contamination of poultry products for human consumption, resulting in one of principal origins of foodborne diseases. One of the current strategies for the control of avian Salmonella is the use of dietary supplementation probiotics. They prevent the intestinal colonization and invasion by enteropathogens. In this work, strains of genera Lactobacillus, Enterococcus and Propionibacterium were assayed in order to design a compatible combination able to maintain the functional property of each microorganism (capacity for compete by nutrients and binding sites with pathogens in intestinal mucosa and/or produce antibacterial substances). Nine strains isolated from gastrointestinal tract of healthy adult birds belonging to species L. johnsonii, L. crispatus, L. reuteri, L. salivarius and P. acidipropionici (10Exp.8 CFU/mL) were studied as probable constituents of a multi-strain formula. Salmonella Enteritidis, Pullorum, Gallinarum and Typhimurium (10Exp.5 CFU/mL) were used as indicators of antimicrobial activity. The compatibility between potential probiotic bacteria was evaluated by joint growth on solid medium and inhibition of the cell free supernatant (CFS) obtained from each strain against the others by agar diffusion technique. The death kinetic of Salmonella from non-neutralized CFS (NNCFS), in LAPTg broth and cecal water medium, was determined by counting viable cells. All bacteria were compatible, except P. acidipropionici LET 107. The NNCFS of L. crispatus CRL 1453, L. johnsonii CRL 1452, L. salivarius CRL 1384 and E. faecium CRL 1385 produced high antagonist action (inhibition halos: 4-5mm). The inhibitory effect of NNCFS from L. johnsonii CRL 1452, which recorded the lowest pH values (3.8 ± 0.1), on Salmonella Enteritidis was remarkable. In addition, the growth of Salmonella Typhimurium and Enteritidis were inhibited at 30 min by NNCFS obtained from L. johnsonii CRL 1452; whereas those NNCFS from L. salivarius CRL 1384 and L. crispatus CRL 1453 only decreased 2 log unit at 60 min for both Salmonella. These strains shown a powerful antagonism on S. Enteritidis and Typhimurium, especially in a similar environment to the intestinal content of birds, where is expected they will exert their beneficial effects. A multivariable analysis allowed select L. johnsonii CRL 1452, L. crispatus CRL 1453, L. salivarius CRL 1384, E. faecium CRL 1385 and P. acidipropionici LET 105 as members of a potential probiotic supplement for chickens.