EFFECT OF YOGURT WITH OR WITHOUT PROBIOTIC ADDITION ON BODY COMPOSITION CHANGES AND IMMUNE SYSTEM IN AN OBESE MODEL.

MALDONADO GALDEANO, CAROLINA; BALCELLS, FLORENCIA; PERDIGÓN GABRIELA

Mar del Plata

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

SAI, SAFE, SAIC, NANOMEDAR,AACYTAL

Aim: to study the effect of feeding with conventional yogurt or yogurt probiotic added on body weight, biochemical parameters, improvement on the intestinal immune system in an obese model.Males adults BALB/c mice were divided into 2 groups: Normal Control (NC) that received a conventional balanced diet and water ad libitum, Obese Control (OC) fed with a high-fat diet. The OC group was divided in sub-groups according to the dietary supplement administered: low-fat milk (OM), conventional yogurt (OY) and probiotic yogurt (OP) for 8 weeks. Body weight was determined 3 times per week. Animals were sacrificed at 30 and 60 days (30d and 60d)and samples were (serum, small intestinal fluid, large intestine(I), liver and spleen), It was determined glucose(GLU), total cholesterol(TC), HDL, triglycerides(TG), IgA+ cells, IL10, INFγ, IL6, IgA, intestinal microbiota and translocation. Results showed that OC and OM reached peak body weight at 4 weeks, while OY and OP after 6 week. Difference in GLU levels were obtained at 30d between NC and OC. TC and HDL were lowering in OY and OP compared to OC and OM at 30d. TG was increased at 30d in NC, OC and OY decreasing at 60d. Enterobacteria population showed significantly increases in OM at 30 and 60d. No change was observed in the translocation of enterobacteria and lactobacilli in spleen. In liver lactobacilli traslocation increased significantly in OM and OY at 30d. Enterobacteria traslocation increase in OM in time assayed. IgA + cells, total IgA and INFγ increased in OY and OP at 30 and 60d being more significant at 60d. IL6 significantly decreased in OC compared with NC at 30 and 60d and the yogurt supplementation normalized IL6 levels. IL10 was significantly decreased in OC and increase with yogurt supplementation. These results show the beneficial effect of yogurt and probiotic yogurt on the intestinal immune response and improvement of the intestinal barrier in obesity, one of the most important chronic diseases.