Release of Cobalamin by Lactobacillus Coryniformis CRL 1001 during the transit trough the gastrointestinal tract

TORINO, MARIA INES; AVILA, ANA MAGDALENA; TORES, ANDREA CAROLINA; TARANTO, MARIA PIA

San Miguel de Tucuman

Simposio; SIBAL 2016 - V Simposio Internacional de Bacterias Lácticas; 2016

CERELA-CONICET

Cobalamin is synthesized only by some bacteria and archaea and is essential for humans who absorbed this vitamin by an intestinal mechanism using intrinsic factor, a protein produced by the stomach cells. We demonstrated that Lactobacillus (L.) coryniformis CRL 1001 genome has at least 30 genes involved in the cobalamin biosynthesis (cbi-cob-hem genes) and the cell extract of this strain is able to correct the coenzyme B12 requirements of Salmonella AR  2680 in a minimal medium. These finding were the first evidence for cobalamin biosynthesis de novo in this specie of Lactobacillus.The aim of this study was to evaluate the survival and cobalamin released by lysed cells of CRL 1001 strain during the exposition to gastrointestinal artificial conditions. A decrease in the cellular viability was observed in the presence of artificial solutions similar to saliva, gastric juice and pancreatic juice. In the presence of supernatant of gastric and pancretic juice inoculated with the L. coryniformis CRL 1001, the AR 2680 strain showed growth halos. These results would bring out that the CRL 1001 strain would be partially lysed during the gastrointestinal passage releasing cobalamin. These results support the idea of using this strain for the development of functional foods for people with vitamin B12 deficiency.