Effect of Ochratoxin A on Cytokines Production By PBMC: Modulation By Probiotic Lactobacilli Strains

MECHOUD, M.; JUAREZ, G.; RODRIGUEZ, A.V.; FONT DE VALDEZ G.

Carlos Paz, Córdoba, Argentina

Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2008

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

EFFECT OF OCHRATOXIN A ON CYTOKINES PRODUCTION BY PBMC: MODULATION BY PROBIOTIC LACTOBACILLI STRAINS Mechoud MA, Juarez GE, Rodriguez AV, Font de Valdez G. CERELA-CONICET. Chacabuco 145. CP 4000. Tucumán, Argentina. E-mail: mmechoud@cerela.org.ar Ochratoxin A (OTA) is a toxin produced by some fungus species and it is commonly found in alimentary products. This toxin is pro-apoptotic and carcinogenic and changes cytokines profile of some cells. Previous studies in our laboratory showed the immunomodulatory capacity of L. acidophilus CRL 1014 and L. reuteri CRL 1098. The aim of this work was to investigate the capacity of these strains to modulate the changes in cytokines induced by OTA in human peripheral blood mononuclear cells (PBMC). 2x106 PBMC were treated with OTA (2-100 μg/ml) at different incubation times at 37ºC and 5% CO2. A pro-inflammatory cytokine (TNF-α) and a regulatory cytokine (IL-10) produced by PBMC were measured by ELISA kit. The lowest concentration of OTA (2 µg/ml) increased TNF-α production (4 times), while higher concentrations (5 to 100 µg/ml) produced an inhibitory effect (43-93%) at 4h incubation. IL-10 was inhibited at all OTA concentrations tested. PBMC exposed to 7.5 μg/ml OTA were co-cultured with L. reuteri or L. acidophilus for 4h. Results showed that L. reuteri increased TNF-α inhibition produced by OTA; in contrast , L. acidophilus reverted the toxin effect. Neither strains modified the inhibition of IL-10 production by OTA. These results are encouraged, indicating that certain lactobacilli strains may protect the host against the adverse health effects of OTA.