SURVIVAL OF BENEFICIAL VAGINAL LACTIC ACID BACTERIA TO GASTROINTESTINAL SIMULATED CONDITIONS

JESSICA SILVA; ANTONELLA MARCHESI; M.E.FATIMA NADER

Rosario-Santa Fe

Congreso; "4ta Reunion Internacional de Ciencias Farmaceuticas (RICIFA)"; 2016

Facultad de Ciencias Bioquímicas y Farmacéuticas - UNR

SURVIVAL OF BENEFICIALVAGINAL LACTIC ACID BACTERIA TO GASTROINTESTINAL SIMULATED CONDITIONSMarchesi Antonella, SilvaJessica, Nader María Elena FátimaCentrode Referencia de Lactobacilos de Tucumán. Chacabuco 145. 4000 Tucumán,Argentina. E_mail: fnader@cerela.org.ar,amarchesi@cerela.org.ar The increase of the knowledge on thevaginal human microbiome, and the dominance of lactobacilli in the humanvaginal-tract encourage the application of vaginal probiotic applications forpreventive and therapeutic goals. Our research group is working on beneficialvaginal-lactic-acid-bacteria (VBLAB) for the design of vaginal pharmabiotic-products.The aim of this work was to evaluate if the VBLAB survive simulatedgastrointestinal conditions, to be later included in the design of an oralformula, on the hypothesis of the ascendant colonization from rectum to thevaginal-tract. The simulated conditions were assayed with different bile saltconcentration, pH and enzymes. The lactobacilli species were: Lactobacillus reuteri, L.gasseri, L.rhamnosus and L.salivarius. Cultures of each strain in the exponential phase were inoculated in microwells plates. The optical density(OD560nm) of the growth was determined at 3,6,9,12 and 24h, withdifferent bile salt concentration (0.25,0.5,1,2%) and pH (3.5,4.5,6.5). Thesimulated tract conditions were performed as follows: the oral cavity enzymes (amylase-lysozyme)were added to bacteria (108CFU/ml) in tubes for 15 minutes. The cellswere added to gastric juice conditions (pepsin-bile salts) for 90 minutes.Later, the bacteria were exposed to pancreatin, chymotrypsin and trypsin during120 minutes. The number of survival bacteria was determined by the successivedilution-method in agar plates. The results obtained show that L.reuteri CRL1327 and CRL1324 were the mostresistant strain to salt and pH with a 25% and 40% decrease of the growth, whileL.rhamnosus CRL1332 and L.salivarius CRL1328 were moresensitive, with a decrease around 65%. The resistance of the strains to the stressful single conditions ofthe gastrointestinal-tract indicates a similar behaviour in the sequentialexperiments with enzymes, low pH and bile salts. The results obtained help us to continue working onthe design of an oral formulation with VBLAB-gut conditions survived- for therestoration of the urogenital-tract microbiome.