CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Antifungal activity of phenolic extracts on yeast contaminants blueberry juice
Autor/es:
CLAUDIA V. VALLEJO; MARÍA J. RODRIGUEZ-VAQUERO; ROLLÁN GRACIELA C
Lugar:
Medellin
Reunión:
Congreso; XIII Congreso Latinoamericano de Microbiología e Higiene de los Alimentos.; 2016
Resumen:
BACKGROUND: The blueberry culture is one of the principal agricultural activities of our province. Previous studies demonstrate that the phenolic compounds of plants have beneficial activities for the consumer. AIM: The aims of this work was the characterization of phenolic compounds profile present in different varieties of blueberries cultivated in Tucuman and the evaluation of their potential antifungal activity against yeast isolated from contaminated strawberry juices. METHODS: The phenolic fraction of Misty, Blue Crisp, O´neal and Millennium varieties of blueberries were extracted using ethyl acetate and phenolic composition was evaluated using TLC, Folin Ciocalteu reagent and HPLC-DAD. The isolation and identification of yeast of deteriorate juice was carried out in YMPG media supplemented with chloramphenicol, phenotypic and genotypic characterization and the phylogenetic tree was constructed. The antifungal activity on isolated yeast was studied by determining the viability and the effect on the synthesis of ergosterol in the presence of phenolic compounds. RESULTS: The phenolic prolife was different between varieties, but chlorogenic acid was the majority compound in all of them. Hanseniaspora osmophila was isolated and identified in deteriorated juice. Phenolic compounds present in blueberries decrease the growth of the yeast, as well as the ergosterol synthesis. CONCLUSIONS: This is the first study about the polyphenol profile differentiation between blueberries varieties and the antifungal activity of these polyphenolics extracts against spoilage yeasts isolated and identified of our region. These results are fundamental to find new strategies for the control of this deleterious yeast.