CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ORAL ADMINISTRAION OF IMMUNOMODULATORY BACTERIUM LIKE PARTICLES ENHANCES MUCOSAL AND SYSTEMIC IMMUNE RESPONSE INDUCED BY ROTAVIRUS ORAL VACCINE
Autor/es:
RAYA-TONETTI, MF; VILLENA, JULIO; RAIMONDO, PAULA; ALVAREZ, SUSANA; SALVA, SUSANA; VIZOSO-PINTO, MARÍA GUADALUPE
Lugar:
San Miguel de Tucumán
Reunión:
Simposio; V Simposio Internacional de Bacterias Lácticas (SIBAL); 2016
Institución organizadora:
Centro de Referencia para Lactobacilos (CERELA-CONICET)
Resumen:
Rotavirus (RVs) is the major causative agent of gastroenteritis in children under 5 years, being a major cause of mortality. There are two oral commercial vaccines for preventing RVs infection: Rotarix (monovalent) and RotaTeq (pentavalent). RVs vaccines have been included into the compulsory vaccination schedule in several countries including Argentina. In order to enhance effectiveness and eventually reduce costs of RVs vaccination, we investigated the adjuvant capacity of Immunomodulatory Bacterium Like Particles (IBLP) obtained by acid-heat treatment of Lactobacillus rhamnosus CRL1505, L. rhamnosus IBL027, L. plantarum CRL1506, or L. paraplantarum CRL1905 co-administered with Rotateq in a murine model. Three-week old Balb/c mice were orally immunized three times every 14 days with 4x104 infecting units/mouse of RotaTeq vaccine (control) or RotaTeq administered with the different IBLP (1x108 particles/mice). Fifteen days after the last immunization, the humoral and cellular immune responses at the mucosal and systemic compartments were evaluated. Orally administered RVs vaccine was able to induce humoral and cellular specific immune responses as evidenced by the detection of specific intestinal and serum IgG, IgA and IgM as well as increased production of IL-4 and IFN-γ by splenocytes and lymphocytes from Payer´s patches after antigen stimulation. The four IBLPs evaluated in this work were able to increase the levels of specific IgA in intestinal fluid (p