NASAL PRIMING WITH IMMUNOBIOTIC Lactobacillus rhamnosus IMPROVES ADAPTIVE IMMUNE RESPONSE AGAINST INFLUENZA VIRUS

TADA, ASUKA; SALVA, SUSANA; VILLENA, JULIO; VIZOSO-PINTO, MARÍA GUADALUPE; MARRANZINO, GABRIELA; KITAZAWA, HARUKI; RAYA-TONETTI, MF; ALVAREZ, SUSANA

San Miguel de Tucumán

Simposio; V Simposio Internacional de Bacterias Lácticas (SIBAL); 2016

Centro de Referencia para Lactobacilos (CERELA-CONICET)

We demonstrated previously that the nasal administration of viable (Lr05) or heat-killed (HK05) Lactobacillus rhamnosus CRL1505 protects mice against influenza virus (IFV) infection by stimulating respiratory innate immune response. In this work, we evaluated whether Lr05 and Hk05 treatments differentially modulated respiratory and systemic anti-IFV adaptive immune response. Six-week-old BALB/c mice were treated with Lr05 or HK05 by the nasal route for 2 days. Treated and untreated control mice were then nasally challenged with IFV. Levels of respiratory and serum IFN-γ, IL-4, IL-17, and IL-10; anti-IFV IgG, IgA, IgG1, and IgG2a; and the number of lung CD4+IL-4+, CD4+IL-17+, and CD4+IFN-+ T cells were evaluated on several days after IFV challenge. As expected, challenge with IFV increased the levels of the four cytokines studied in serum and the respiratory tract as well as the number of lung IL-4, IL-17, and IFN- producing T cells. In addition, serum and respiratory anti-IFV antibodies were detected from day 5 post-infection and the levels of IgA, IgG, IgG1, and IgG2a increased until day 15. Both, Lr05 and HK05 significantly increased the numbers of lung CD4+IFN-+ T cells, and the concentration of IFN- and IL-10 when compared to controls, being Lr05 more effective than HK05 to induce those effects (p