CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
COMPARATIVE in vivo AND in vitro STUDY OF IMMUNOMODULATORY PROPERTIES OF PEPTIDOGLYCANS OBTAINED FROM DIFFERENT Lactobacillus STRAINS
Autor/es:
SALVA, SUSANA; ALVAREZ, SUSANA; FERRERO, MARIANA; BALDI, PABLO; KOLLING, YANINA; VILLENA, JULIO
Lugar:
San Miguel de Tucumán
Reunión:
Simposio; V Simposio Internacional de Bacterias Lácticas (SIBAL); 2016
Institución organizadora:
Centro de Referencia para Lactobacilos (CERELA-CONICET)
Resumen:
The use of non-viable lactic acid bacteria and their cellular fractions are excellent alternatives to stimulate immunity in immunocompromised hosts, in which viable microorganisms could involve a health risk. The aim of this work was to compare the immunomodulatory properties of the peptidoglycan obtained from the immunobiotic Lactobacillus rhamnosus CRL1505 (Pg05), with the peptidoglycans obtained from the non-probiotic L. rhamnosus CRL534 (Pg534) and from the probiotic L. plantarum CRL1506 (Pg06) by in vitro and in vivo studies. First, we studied the effect on the innate immune response in immunocompromised-malnourished mice. Weaned mice were malnourished with a protein-free diet for 21 days and then received balanced diet during 7d (BD group) or were fed BD 7d with nasal supplementation of Pg05, Pg06 or Pg534 during the last 2d. On d8 were infected all experimental groups with Streptococcus pneumoniae (Sp) and the resistance to the infection and innate immune response were studied. Lung and blood Sp counts in Pg06 and Pg534 groups were similar to the BD group while the pathogen was not detected in samples of Pg05-treated mice. Moreover, lung tissue injuries in Pg05 were significantly lower when compared to the other groups. Pneumococcal infection increased CD45+Gr-1+ neutrophils, CD45+F4/80+CD11b-CD11c+ macrophages, TNF-α and IL-10 in the respiratory tract of all groups. However, the increment of phagocyte numbers as well as their MHC-II expression were higher in Pg05 mice than on the other groups. In addition, Pg05 mice showed a lower TNF-α/IL-10 ratio when compared to Pg06 and Pg534 groups. In order to understand the mechanisms involved in the differential immune responses induced by the peptydoglicans obtained from different strains of lactobacilli, we studied the interactions with mice lung epithelial cells (LEC) or alveolar macrophages (AM) using in vitro systems. In this way, LEC or AM were cultured 24 h in the absence or presence of S. pneumoniae with or without the addition of Pg05, Pg06 or Pg534. We demonstrated that 90% of LEC and AM remained viable after the treatments with Pgs. Pg05 (50 ug/mL) significantly modulated the activation of AM by an increase of IL-1β and KC (p