Immunomodulation of Lactobacillus rhamnosus RC007 on the intestinal toxicity of deoxynivalenol

G. GARCIA; C. GRECO; L. CAVAGLIERI; C. DOGI; A. DE MORENO DE LEBLANC; D. PAYROS; S.N. CHULZE; I.P. OSWALD

Amsterdam

Conferencia; The fifth Beneficial Microbes Conference - Pre- and probiotics for lifelong human and animal health.; 2016

Beneficial Microbes

The legislation of the European Union banned antibiotics as growth promoters in food animals. In addition, the Food and Agricultural Organization estimates that mycotoxins contaminate 25% of the world?s agricultural commodities. Different alternatives, such as the use of probiotics, have been explored to stimulate gut health in weaned pigs. Lactobacillus rhamnosus RC007 was previously isolated from maize silage and demonstrated beneficial properties in a mice colitis model. The aim of the present study was to evaluate the immune effect on the intestinal toxicity of deoxynivalenol (DON) in an ex vivo porcine model. Jejunal explants were obtained from 5 weeks old crossbred castrated male piglets. The treatments evaluated were: 1) porcine jejunal explants in complete medium, 2) porcine jejunal explants exposed for 3 h to10 μM DON in complete medium, 3) porcine jejunal explants with 109 UFC/ml L. rhamnosus RC007 in complete medium, 4) porcine jejunal explants pre-incubated 1 h with 109 UFC/ml L. rhamnosus RC007 and then exposed for 3 h to 10 μM DON in complete medium. The explants were incubated at 39°C. For the gene expression analysis, total RNAs were extracted and then reverse transcription and qPCR steps were performed to determinate IL-1β, TNFα, IL-8 and IL-22. Cellular permeability was measured by a Ussing chamber. Treatment of intestinal explants with DON significantly increased the expression of IL-1β, TNFα, IL-8, IL-22 and increased the paracellular permeability. By contrast, the pre-incubation with L. rhamnosus RC007 reduced the expression of these pro-inflammatory cytokines and the paracellular permeability. In conclusion, L. rhamnosus RC007 is a probiotic candidate to be included as feed additive able to decreases the intestinal toxicity caused by DON.