FERULOYL ESTERASE ACTIVITY IS INFLUENCED BY BILE, PROBIOTIC INTESTINAL ADHESION AND MILK COMPONENTS

ABEIJÓN MUKDSI, CLAUDIA; ARGAÑARAZ MARTÍNEZ, ELOY; MEDINA, ROXANA; PÉREZ CHAIA, ADRIANA

Mar del Plata

Jornada; LI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2015

Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

Feruloyl esterases (FE) are bacterial and mammalian enzymes that catalyze the hydrolysis of hydroxycinnamate esters releasing antioxidant hydroxycinnamic acids. Administration of Lactobacillus fermentum (Lf) CRL1446, FEproducing strain, increased intestinal FE activity and improved oxidative status in mice. The aim of this work was to evaluate the effects of bile, adhesion to intestinal epithelium and milk components on FE activity. Effect of bile was evaluated by incubation of cells in PBS+Oxgall. FE activity was determined in supernatants and cells by incubation in PBS containing 1 mM methyl ferulate. Released HA was quantified by HPLC. Adhesion was evaluated ex vivo by incubation of Lf suspensions (109 cfu/ml) with intestinal tissue fragments (ITF) and exfoliated intestinal epithelial cells (IEC). Effect of milk components on FE activity was assessed by incubation of ITF (with/without adhered Lf) in presence of milk fat and calcium salts. Incubation with bile increased FE activity in whole cells and supernatants (2? fold), compared to controls. Lf was able to adhere to ITF (106 cfu/mm2 ), and when evaluated in IEC, a 17% adhesion index was observed. Higher activities were detected in ITF with adhered bacteria and incubated in presence of milk fat and calcium salts. These results support the potential use of L. fermentum CRL1446 for the development of new functional foods.