Bifidobacterium enzymes involved in lipids metabolism: impact on the development of functional foods

TERAN, V; MEDINA, R; VAN NIUEWENHOVE, C

Mar del Plata

Congreso; LI Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2015

Few studies showed the influence of bifidobacteria over lipids derived from milk products, especially fatty acids (FA) hydrolysis by esterase and lipases enzymes and production of CLA and CLNA by isomerases with health-promoting properties. The aim of this work was to determine enzymes activities by 3 bifidobacteria: INL2, ZL1228 and LM7a, isolated from breast milk and cultured in MRS-cys at 37 °C, 48 h under anaerobic conditions. Esterase activity was measured in cell-free extracts by PAGE using acrylamide gels at 12% without SDS and incubated in presence of α-naphtil derivates from FA (C2-C12) and Fast Red TR as developer. To evaluate bioactive lipids production by GC bacteria were cultured in MRS-cys and skim milk 10% with LA and LNA. The highest level of esterase activity was detected against α-naphtil acetate, propionate y butyrate. Low levels were obtained in presence of C10 and 12 for LM7a and ZL1228. Electrophoretic zymograms showed 4 esterase enzymes with different electrophoretic mobilities and substrate specificity. No lipase activity was detected. All strains produce CLA in MRS-cys and skim milk. INL2 produced the highest levels of CLNA in MRS-cys while ZL1228 resulted the best producer in skim milk. This study shows bifidobacterias with esterase and isomerase activity, both important for the development of functional foods with desirable organoleptic characteristics.