CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A simplified culture medium for an anti-inflammatory soluble factor production by L. reuteri CRL 1098
Autor/es:
GRIET M; LANZA L; CAZORLA SI; GEREZ CL; FONT DE VALDEZ G; RODRÍGUEZ AV
Lugar:
Mar del Plata
Reunión:
Congreso; LI Reunión SAIB 2015; 2015
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
Some specific soluble factors secreted by probiotic lactic acid bacteria are able to modulate the immune response. Our previous studies demonstrated that a Lactobacillus reuteri CRL 1098 soluble factor identified as a 5785 Da peptide, present in the supernatant (LrS), modulates the inflammatory response triggered by LPS in vitro and in a mice model. LrS was produced under stress conditions by starved cells after 4h incubation in RPMI 1640 medium, where the bacteria maintained alive but did not proliferate. The aim of this work was to improve LrS production. Two steps will be performed: 1) Design of a simplified and low cost medium for optimal LrS production. Casitone medium, PBS, TRIS, citrate buffer with and without glucose were assayed. Bacteria survival was evaluated by cell counting in MRS agar. LrS cytotoxicity was determined by Trypan blue and MTT assay. L. reuteri only maintained alive in casitone medium and citrate buffer with 4 g/l glucose. These LrS diminished TNF-α and nitric oxide production in both mouse peritoneal macrophages and RAW 264.7 cells stimulated with LPS. 2) Optimization of medium components (MRS) for L. reuteri biomass production, using a factorial statistical experimental model, is in progress. Application of an economic and more competitive medium for LrS production by L. reuteri CRL 1098 represents an important step for its enforcement at pilot scale.