Effect of Saccharomyces cerevisiae RC016 on s-Iga and microbiota in piglets for the formulation of feed additives

L. CAVAGLIERI; C. DOGI; A. FOCHESATO; C. GRECO; A. DE MORENO DE LEBLANC

Congreso; 11 SLACA. Latin American Symposium of Food Science - "Food Science: Quality of Life and Healthy Ageing.; 2015

SLACA

Concerns regarding resistance in human bacterial pathogens have led to the total ban of antibiotics as growth promoters in animal feed. In recent years, different alternatives such as probiotics, have been explored and represent a potentially significant prophylactic or therapeutic safe advance to control enteric bacterial diseases. The aim was to evaluate the effect in vivo of Saccharomyces cerevisiae RC016 on Secretory IgA (s-IgA) levels and microbiota of gut in piglets.A total of six pigs were weaning at 21 days of age and assigned to two groups: Control Group (CG) and Yeast Group (YG). Animals received S. cerevisiae RC016 during three weeks and maintained in an extensive outdoor system. Feed and water were available ad libitum.Oral administration of S. cerevisiae RC016 increased s-IgA in small intestine (CG: 145.81 ± 17.43 μg/ml vs YG:219.04 ± 46.25 μg/ml and the count of lactobacilli in large intestine (CG: 5.50±0.17 log UFC/g vs YG:6.16 ± 0.19 log UFC/g ). The administration of the yeast did not affect the count of enterobacteriacea (CG:3.29±0.70 log UFC/g vs YG: 3.44±0.73 log UFC/g). The s-IgA is the main mechanism of protection given by the gut associated lymphoid tissue that prevents the entry of potentially harmful antigens and also interacts with mucosal pathogens without potentiating damages. Therefore the stimulation of s-IgA is often considered a desirable property in the screening of probiotic microorganisms. Lactobacilli and enterobacteria have been traditionally selected as microbial groups with a particular significance for gut health. In the present work, the administration of the yeast increase lactobacilli and its counts were higher than obtained for enterobacteria. The presence of lactobacilli in the gastrointestinal tract of pigs is benefical, whereas the presence of enterotoxigenic coli bacteria is associated with diarrhea and other disorders. Lactobacilli could have a predominant role in controlling colibacillosis, one of the most common intestinal disorders during the first months of life. In conclusion, the modulation immune of IgA and gut microbiota observed showed that S. cerevisiae RC016 is a promising candidate for feed additives formulation to improve animal productivity.