CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development of an Andean potato product with folate-producing lactic acid bacteria
Autor/es:
MOSSO, A.; JIMENEZ, E.; VIGNOLO, G.; LEBLANC, JEAN GUY; SAMMAN, N.
Reunión:
Congreso; 8th Probiotic, Prebiotic and New Food for Microbiota and Human Health; 2015
Resumen:
Folate is a B-group vitamin that cannot be synthesized by humans and must be obtained exogenously. It is known that certain lactic acid bacteria (LAB) strains can produce this vitamin. The aim of the study was to select folate-producing LAB in order to obtain a novel fermented potato product with elevated folate levels. Several LAB strains were isolated from Tocosh (a traditional Andean fermented potato product) and cultured in folate-free culture medium (FACM) The LAB which produced the highest amounts of folated (determined by a microbiological method) were selected for the fermentation of two Andean potato varieties (S. tuberosum ssp andigena churqueña and collareja). Sterile cooked purees were inoculated and samples were collected at 0, 8 and 24 hours. Cell growth, pH and total folate were determined. From a total of 63 tested strains, 40 could grow in FACM after 7 subcultures. Lactobacillus (Lb.) sakei 2T2MM10 and 2T3MS8, Lb. fermentum T3M3, Lb. paracasei 3T3M3 and 3T3MS2 produced the highest folate concentrations in FACM (between 29 and 138 ng/ml). These were used to ferment the two potato varieties. Although all 5 selected strains were able to grow and produce folates in the potatoes, L. sakei 2T3MS8 showed the highest increase after 8h incubation, with folate concentrations reaching between 300-400 ng/g which is more than 3 fold the amounts of the unfermented potatoes (100±30ng/g).Conclusion: The adequate selection of LAB could be used to produce novel potato based products with elevated folate concentrations.