CERELA   05438
CENTRO DE REFERENCIA PARA LACTOBACILOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genotypic identification of Lactic Acid Bacteria (LAB) from a Rana catesbeiana hachery
Autor/es:
SERGIO E PASTERIS; A. GONZALEZ; P. VANDAMME; A. VAN SCHOOR; M.I. BUHLER; M.E. NADER-MACIAS; L. DE VUYST
Lugar:
Rosario, Santa Fe
Reunión:
Congreso; V Congreso Argentino de SAMIGE; 2008
Institución organizadora:
SAMIGE
Resumen:
Raniculture has grown substantially due to the increasing demand for frog´s meat and by-products. Farming operations require frogs to be placed in confinement which increases the risk of epizootics, such as the red-leg syndrome (RLS). RLS is the main cause of mortality and significant economic loses in raniculture. Currently, the prevention and control of aquaculture diseases have focused on good husbandry practices and the use of vaccines or antibiotics. Treating or feeding frogs with antibiotics may cause the development of resistant bacteria. Thus, the use of probiotics represents an alternative method of prevention. The use of probiotics requires a correct identification of the strains, mainly because aquaculture products are used for human consumption. Lactic acid bacteria (LAB) isolated from the indigenous microbiota of a R. catesbeiana hatchery from Argentina were identified by phenotipic tests, and show some beneficial properties. Therefore, the aim of this work was to apply DNA- and RNA-based methodologies to identify the isolates, through a combination of (GTG)5-PCR fingerprinting, Multi Locus Sequence Analysis, and 16S rDNA gene sequencing analysis. Most of the LAB strains in the population was identified as Pediococcus pentosaceus and Lactobacillus plantarum, although single clusters of Lb. curvatus, Lactococcus lactis, Leuconostoc mesenteroides subsp. cremoris, Enterococcus faecium, and Ent. faecalis were also found. Moreover, some strains remain unidentified and they could belong to new LAB species. The present work is the first assessment of the genetic identification of this specific group of microorganisms in raniculture. In this field, the use of LAB as biological control agents represents an interesting alternative to avoid the use of antibiotics as it has been reported in fish aquaculture.